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KMID : 1094719970020010015
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Biotechnology and Bioprocess Engineering
1997 Volume.2 No. 1 p.15 ~ p.18
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Expression of the IL-2 gene in the potato (Solanum tuberosum cv. Superior)
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Park Yoon-Kyung
Hwang Baik
Pyo Byoung-sik
Cheong Hyeon-Sook
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Abstract
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We intended to examine the expression of the T-cell growth factor (human interleukin-2) so that a binary vector, pSSK-1, carrying the IL-2 gene was constructed and transferred intoA. tumefaciens for the purpose of the transformation of the potato (Solanum tuberosum cv. Superior). All of theAgrobacterium-infected potato explants were regenerated to shoots in modified MS medium and 81% of them rooted on the medium containing kanamycin (200 mg/L). Southern and Northern analysis were performed to verify the transformation events. EL-ISA test indicated that IL-2 protein was produced from IL-2-transformed potatoes. These results suggested expression and production of the IL-2 protein from the transgenic potato.
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KEYWORD
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T-cell growth factor, IL-2 protein, potato (Solanum tuberosum cv. Superior), modified MS medium, expression of the IL-2 gene
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